Magnetic Resonance Imaging
Volume 24, Issue 5 , Pages 611-617 , June 2006

In vitro labeling and MRI of mesenchymal stem cells from human umbilical cord blood

  • Shenghong Ju

      Affiliations

    • Laboratory of Molecular Imaging, Department of Radiology, Zhongda Hospital, Southeast University, Nanjing 210009, China
  • ,
  • Gaojun Teng

      Affiliations

    • Laboratory of Molecular Imaging, Department of Radiology, Zhongda Hospital, Southeast University, Nanjing 210009, China
    • Corresponding Author InformationCorresponding author. Tel.: +86 25 83272121; fax: +86 25 83311083.
  • ,
  • Yu Zhang

      Affiliations

    • Laboratory of Molecular and Boimolecular Electronics, Southeast University, Nanjing 210009, China
  • ,
  • Ming Ma

      Affiliations

    • Laboratory of Molecular and Boimolecular Electronics, Southeast University, Nanjing 210009, China
  • ,
  • Feng Chen

      Affiliations

    • Laboratory of Molecular Imaging, Department of Radiology, Zhongda Hospital, Southeast University, Nanjing 210009, China
    • Department of Radiology, University Hospitals, K.U.LEUVEN, B-3000 Leuven, Belgium
  • ,
  • Yicheng Ni

      Affiliations

    • Department of Radiology, University Hospitals, K.U.LEUVEN, B-3000 Leuven, Belgium

Received 16 September 2005 ,Accepted 17 December 2005.

References 

  1. Bulte JW, Douglas T, Witwer B, Zhang SC, Lewis BK, van Gelderen P, et al. Monitoring stem cell therapy in vivo using magnetodendrimers as a new class of cellular MR contrast agents. Acad Radiol. 2002;9(Suppl 2):S332–S335
  2. Bulte JW, Zhang SC, van Gelderen P, Herynek V, Jordan EK, Janssen CH, et al. Magnetically labeled glial cells as cellular MR contrast agents. Acad Radiol. 2002;9(Suppl 1):S148–S150
  3. Modo M, Cash D, Mellodew K, Williams SC, Fraser SE, Meade TJ, et al. Tracking transplanted stem cell migration using bifunctional, contrast agent-enhanced, magnetic resonance imaging. NeuroImage. 2002;17:803–811
  4. Weissleder R. Molecular imaging: exploring the next frontier. Radiology. 1999;212:609–614
  5. Unger EC. How can superparamagnetic iron oxides be used to monitor disease and treatment?. Radiology. 2003;229:615–616
  6. Bicknese AR, Goodwin HS, Quinn CO, Henderson VC, Chien SN, Wall DA. Human umbilical cord blood cells can be induced to express markers for neurons and glia. Cell Transplant. 2002;11:261–264
  7. Deans RJ, Moseley AB. Mesenchymal stem cells: biology and potential clinical uses. Exp Hematol. 2000;28:875–884
  8. Erices A, Conget P, Minguell JJ. Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol. 2000;109:235–242
  9. Ma M, Zhang Y, Yu W, Shen H, Zhang H, Gu N. Preparation and characterization of magnetite nanoparticles coated by amino silane. Coll Surf. 2003;212:219
  10. Ma M, Wu Y, Zhou J, Sun Y, Zhang Y, Gu N. Size-dependence of specific power absorption of Fe3O4 particles in AC magnetic field. J Magn Magn Mater. 2003;268:33–39
  11. Liu L, Tang M, Gu N, Xiong L, Ma M, Zhang Y, et al. Study on acute and chronic toxicity to rats of nanoparticles of Fe2O3 coated with glutamic acid. J Environ Occup Med. 2004;21:430–433[in Chinese]
  12. Li Q, Tang M, Ban T, Ma M, Gu N. Study on cytotoxicity and oxidative effects of different sizes of Fe2O3 nanoparticles on CHL cells in vitro. Chin J Public Health. 2005;21:589–591[in Chinese]
  13. Yin Q, Liu L, Gu N, Huang Y, Liu L, Song J, et al. Determining the biodistribution of nano-Fe2O3-Glu in mice by 59Fe tracer and preparation. J Med Postgrad. 2005;18:312–317[in Chinese]
  14. Yeh TC, Zhang W, Ildstad ST, Ho C. In vivo dynamic MRI tracking of rat T-cells labeled with superparamagnetic iron-oxide particles. Magn Reson Med. 1995;33:200–208
  15. Josephson L, Tung CH, Moore A, Weissleder R. High-efficiency intracellular magnetic labeling with novel superparamagnetic-tat peptide conjugates. Bioconjug Chem. 1999;10:186–191
  16. Daldrup-Link HE, Rudelius M, Oostendorp RA, Settles M, Piontek G, Metz S, et al. Targeting of hematopoietic progenitor cells with MR contrast agents. Radiology. 2003;228:760–767
  17. Weissleder R, Stark DD, Engelstad BL, Bacon BR, Compton CC, White DL, et al. Superparamagetic iron oxide: pharmacokinetics and toxicity. AJR Am J Roentgenol. 1989;152:167–173
  18. Bulte JW, Zhang S, van Gelderen P, Herynek V, Jordan EK, Duncan ID, et al. Neurotransplantation of magnetically labeled oligodendrocyte progenitors: magnetic resonance tracking of cell migration and myelination. Proc Natl Acad Sci U S A. 1999;96:15256–15261
  19. Lewin M, Carlesso N, Tung CH, Tang XW, Cory D, Scadden DT, et al. Tat peptide-derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells. Nat Biotechnol. 2000;18:410–414
  20. Bulte JW, Douglas T, Witwer B, Zhang SC, Strable E, Lewis BK, et al. Magnetodendrimers allow endosomal magnetic labeling and in vivo tracking of stem cells. Nat Biotechnol. 2001;19:1141–1147
  21. Frank JA, Miller BR, Arbab AS, Zywicke HA, Jordan EK, Lewis BK, et al. Clinically applicable labeling of mammalian and stem cells by combining superparamagnetic iron oxides and transfection agents. Radiology. 2003;228:480–487
  22. Arbab AS, Bashaw LA, Miller BR, Jordan EK, Lewis BK, Kalish H, et al. Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging. Radiology. 2003;229:838–846
  23. Wilhelm C, Billotey C, Roger J, Pons JN, Bacri JC, Gazeau F. Intracellular uptake of anionic superparamagnetic nanoparticles as a function of their surface coating. Biomaterials. 2003;24:1001–1011
  24. Frank JA, Zywicke H, Jordan EK, Mitchell J, Lewis BK, Miller B, et al. Magnetic intracellular labeling of mammalian cells by combining (FDA-approved) superparamagnetic iron oxide MR contrast agents and commonly used transfection agents. Acad Radiol. 2002;9(Suppl 2):S484–S487
  25. Hoehn M, Kustermann E, Blunk J, Wiedermann D, Trapp T, Wecker S, et al. Monitoring of implanted stem cell migration in vivo: a highly resolved in vivo magnetic resonance imaging investigation of experimental stroke in rat. Proc Natl Acad Sci U S A. 2002;99:16267–16272
  26. Kraitchman DL, Heldman AW, Atalar E, Amado LC, Martin BJ, Pittenger MF, et al. In vivo magnetic resonance imaging of mesenchymal stem cells in myocardial infarction. Circulation. 2003;107:2290–2293
  27. Hill JM, Dick JA, Raman VK, Thompson RB, Yu ZX, Hinds KA, et al. Serial cardiac magnetic resonance imaging of injected mesenchymal stem cells. Circulation. 2003;108:1009–1014

 Supported by NSFC (National Nature Science Foundation of China), 30400116.

PII: S0730-725X(06)00003-8

doi: 10.1016/j.mri.2005.12.017

Magnetic Resonance Imaging
Volume 24, Issue 5 , Pages 611-617 , June 2006