Analysis of hyperpolarized dynamic 13C lactate imaging in a transgenic mouse model of prostate cancer

Lupo, Janine M.; Chen, Albert P.; Zierhut, Matthew L.; Bok, Robert A.; Cunningham, Charles H.; Kurhanewicz, John; Vigneron, Daniel B.; Nelson, Sarah J.

doi:10.1016/j.mri.2009.07.007

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Volume 28, Issue 2 , Pages 153-162, February 2010

Analysis of hyperpolarized dynamic ¹³C lactate imaging in a transgenic mouse model of prostate cancer ☆

Janine M. Lupo
- Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA 94158, USA
- Corresponding author. UCSF Radiology, Box 2532, San Francisco, CA 94158-2532, USA. Tel.: +1 415 514 4420; fax: +1 415 514 2550.
Albert P. Chen
- Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA 94158, USA
Matthew L. Zierhut
- Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA 94158, USA
- UCSF/UCB Joint Graduate Group in Bioengineering, University of California San Francisco, San Francisco, CA 94158, USA
Robert A. Bok
- Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA 94158, USA
Charles H. Cunningham
- Department of Medical Biophysics, Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada M56 2M9
John Kurhanewicz
- Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA 94158, USA
- Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA 94158, USA
Daniel B. Vigneron
- Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA 94158, USA
- Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA 94158, USA
Sarah J. Nelson
- Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA 94158, USA
- Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA 94158, USA

Received 5 February 2009; received in revised form 12 May 2009; accepted 4 July 2009. published online 20 August 2009.

Abstract

This study investigated the application of an acquisition that selectively excites the [1-¹³C]lactate resonance and allows dynamic tracking of the conversion of ¹³C-lactate from hyperpolarized ¹³C-pyruvate at a high spatial resolution. In order to characterize metabolic processes occurring in a mouse model of prostate cancer, 20 sequential 3D images of ¹³C-lactate were acquired 5 s apart using a pulse sequence that incorporated a spectral–spatial excitation pulse and a flyback echo-planar readout to track the time course of newly converted ¹³C-lactate after injection of prepolarized ¹³C-pyruvate. The maximum lactate signal (MLS), full-width half-maximum (FWHM), time to the peak ¹³C-lactate signal (TTP) and area under the dynamic curve were calculated from the dynamic images of 10 TRAMP mice and two wild-type controls. The regional variation in ¹³C-lactate associated with the injected pyruvate was demonstrated by the peak of the ¹³C-lactate signal occurring earlier in the kidney than in the tumor region. The intensity of the dynamic ¹³C-lactate curves also varied spatially within the tumor, illustrating the heterogeneity in metabolism that was most prominent in more advanced stages of disease development. The MLS was significantly higher in TRAMP mice that had advanced disease.

Keywords: DNP, Hyperpolarized ¹³C, Lactate imaging, TRAMP, Prostate cancer

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This article was presented in part at the 16th Annual Meeting of ISMRM, Toronto, Ontario, Canada, 2008.

☆ This research was supported by NIH grants R01-EB007588, R21-EB005363 and R01-CA111291, and UC Discovery grants LSIT01-10107 and ITL-BIO04-10148 in conjunction with GE Healthcare.

PII: S0730-725X(09)00180-5

doi:10.1016/j.mri.2009.07.007

« Previous Next »Magnetic Resonance Imaging
Volume 28, Issue 2 , Pages 153-162, February 2010

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