Magnetic Resonance Imaging
Volume 28, Issue 4 , Pages 599-606, May 2010

Quantitative MR imaging of targeted SPIO particles on the cell surface and comparison to flow cytometry☆

  • Kersten Peldschus

      Affiliations

    • Department of Diagnostic and Interventional Radiology, University Cancer Center Hamburg-Eppendorf (UCCH), 20246 Hamburg, Germany
    • Corresponding Author InformationCorresponding author. Tel.: +49 40 42803 3116; fax: +49 40 42803 6799.
    • Both authors contributed equally.
    • ,
  • Alexander Schultze

      Affiliations

    • Department of Hematology and Oncology, University Cancer Center Hamburg-Eppendorf (UCCH), 20246 Hamburg, Germany
    • Both authors contributed equally.
    • ,
  • Peter Nollau

      Affiliations

    • Department of Clinical Chemistry, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany
    • ,
  • Michael Kaul

      Affiliations

    • Department of Diagnostic and Interventional Radiology, University Cancer Center Hamburg-Eppendorf (UCCH), 20246 Hamburg, Germany
    • ,
  • Udo Schumacher

      Affiliations

    • Department of Anatomy II: Experimental Morphology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany
    • ,
  • Christoph Wagener

      Affiliations

    • Department of Clinical Chemistry, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany
    • ,
  • Gerhard Adam

      Affiliations

    • Department of Diagnostic and Interventional Radiology, University Cancer Center Hamburg-Eppendorf (UCCH), 20246 Hamburg, Germany
    • ,
  • Harald Ittrich

      Affiliations

    • Department of Diagnostic and Interventional Radiology, University Cancer Center Hamburg-Eppendorf (UCCH), 20246 Hamburg, Germany

Received 7 October 2009; received in revised form 27 November 2009; accepted 7 December 2009. published online 01 February 2010.

Abstract 

Purpose

To detect anti-CEACAM5 targeted superparamagnetic iron oxide (SPIO) particles in vitro on the cell surface by quantitative magnetic resonance (MR) imaging and to compare with flow cytometry.

Materials and Methods

The monoclonal mouse antibody T84.1 and an appropriate IgG isotype antibody were conjugated to dextran-coated SPIO particles. HT29 cells expressing carcinoembryonic antigen (CEACAM5) were treated with antibody-conjugated SPIO particles. Purified cell samples were examined on a 3.0-T MR scanner using a multi-echo spin-echo sequence for MR relaxometry. Aliquots of the cell samples were further treated with a fluorescein isothiocyanate (FITC) anti-dextran antibody and an Alexa Fluor 488 anti-mouse antibody for the corresponding flow cytometry.

Results

MR relaxometry revealed a dose-dependent binding of T84.1-conjugated SPIO particles with a positive correlation between R2 relaxation rate of cell samples and SPIO particle concentration during incubation (r=0.993, P<.01). Positive correlations were also observed between R2 relaxation rate and flow cytometry (geometric mean) with both fluorescent antibodies (r=0.972 and r=0.953, both P<.01), respectively.

Conclusion

The study revealed the feasibility of quantitative MR imaging of targeted SPIO particles on the cell surface comparable to flow cytometry.

Keywords: Superparamagnetic iron oxide particle, Magnetic resonance relaxometry, Flow cytometry, Carcinoembryonic antigen

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☆ The study was supported by the Molecular Imaging North (MOIN) initiative granted by the State Department of Science and Research of the city and state of Hamburg, Germany. Alexander Schultze was supported by Werner-Otto-Stiftung, Hamburg, Germany.

PII: S0730-725X(09)00309-9

doi:10.1016/j.mri.2009.12.017

Magnetic Resonance Imaging
Volume 28, Issue 4 , Pages 599-606, May 2010

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